Identification of Serratane Synthase Gene from the Fern Lycopodium clavatum.

TitleIdentification of Serratane Synthase Gene from the Fern Lycopodium clavatum.
Publication TypeJournal Article
Year of Publication2017
AuthorsSaga, Y., Araki, T., Araya, H., Saito, K., Yamazaki, M., Suzuki, H., & Kushiro, T.
JournalOrganic letters
Volume19
Issue3
Pagination496-499
Date Published2017 Feb 03
ISSN1523-7052
Abstract

Ferns are known to produce onoceroids including onoceranes and serratanes having unusual structures among triterpenes. From the fern Lycopodium clavatum, a novel onoceroid synthase gene was cloned that showed high sequence identity with a previously identified α-onocerin synthase. Functional analysis by coexpression with pre-α-onocerin synthase in yeast led to the production of tohogenol and serratenediol. The result suggested that serratanes are directly biosynthesized from pre-α-onocerin and not from α-onocerin as previously assumed.

DOI10.1021/acs.orglett.6b03659
Alternate JournalOrg. Lett.